The bus was carrying 51 passengers from Cincinnati to Detroit when it ran off Interstate 75 just north of Cincinnati.
For the second day in a row, a DDOT bus has been involved in an accident.
Six students were taken to the hospital with minor injuries after police say a woman failed to obey a yield sign causing an accident in Detroit.
The accident happened on East Commerce Road near Duck Lake Road, in Milford Township.
WWJ’s Gary Lundy reported one of the cars is believed to have cut off the D-Dot bus causing the accident.
An investigation into a fiery crash between a tractor-trailer and a tour bus that killed the Michigan truck driver and injured at least 30 passengers will focus on just where the bus was when it was hit and how fast it was going.