Michigan Tech Prof's Plants, Bacteria May Give UP's Gay Stamp Sands New Life

HOUGHTON (WWJ) -- The tiny town of Gay on northern Michigan's Keweenaw Peninsula is famous for more than its eponymous Gay Bar.

A short distance away on the shore of Lake Superior is the Gay Stamp Sands wasteland -- miles of shoreline sand that's actually crushed copper-bearing rock, left there by a 19th Century copper industry that operated long before environmental regulation. The sand still contains traces of copper, arsenic and other metals, meaning plants won't grow there. If it wasn't for the blue sky and the blue water, you'd swear you were on the moon.

Now, a professor at nearby Michigan Technological University is experimenting with bacteria and plants to remediate this pollution -- and perhaps bring new life to the stamp sands coastline.

Ramakrishna Wusirika, an associate professor of biological sciences, first collected samples of the stamp sands. Then, Wusirika and his team planted maize in the stamp sand, incorporating bacteria in four different ways: mixing it in the stamp sand before planting seed; coating seed with bacteria and planting it; germinating seeds and planting them in soil to which bacteria were added; and the conventional method, immersing the roots of maize seedlings in bacteria and planting them in stamp sand.

After 45 days, the team uprooted the plants and measured their dry weight. All maize grown with bacteria was significantly more vigorous -- from two to five times larger -- than the maize grown in stamp sand alone. The biggest were those planted as seedlings or as germinated seeds.

However, when the researchers analyzed the dried maize, they made a surprising discovery: the seed-planted maize took up far more copper as a percentage of dry weight. In other words, the smaller plants pulled more copper, ounce per ounce, out of the stamp sands than the bigger ones.

That has implications for land managers trying to remediate contaminated sites, or even for farmers working with marginal soils, Wusirika said. The usual technique -- applying bacteria to seedlings' roots before transplanting -- works fine in the lab but would be impractical for large-scale projects. This could open the door to simple, practical remediation of copper-contaminated soils.

But the mere fact that all the plants grown with bacteria did so well also piqued his curiosity.

"When we saw this, we wondered what the bacteria were doing to the soil," Wusirika said. "Based on our research, it looks like they are improving enzyme activity and increasing soil fertility," in part by freeing up phosphorus that had been locked in the rock.

The bacteria are also changing copper into a form that the plants can take up.

"With bacteria, the exchangeable copper is increased three times," he said. "There's still a lot of copper that's not available, but it is moving in the right direction."

By analyzing metabolic compounds, the team was able to show that the bacteria enhance photosynthesis and help the plants make growth hormones. Bacteria also appear to affect the amount of phenolics produced by the maize. Phenolics are antioxidants similar to those in grapes and red wine.

Compared to plants grown in normal soil without bacteria, plants grown in stamp sand alone showed a five-fold increase in phenolics. However, phenolics in plants grown in stamp sand with bacteria showed a lesser increase.

"Growing in stamp sand is very stressful for plants, and they respond by increasing their antioxidant production," Wusirika said. "Adding the metal-resistant bacteria enables the plants to cope with stress better, resulting in reduced levels of phenolics."

Wusirika noted that "there's still a lot to understand here. We'd like to do a study on stamp sands in the field, and we'd also like to work with plants besides maize. We think this work has applications in organic agriculture as well as remediation."

Wusirika's work is featured on the research crowdfunding site Superior Ideas.

An article on the work, "Integrated Metabolomic and Proteomic Approaches Dissect the Effect of Metal-Resistant Bacteria on Maize Biomass and Copper Uptake," coauthored by PhD graduate student Kefeng Li, now at the University of California at San Diego; graduate students Venkataramana R. Pidatala and Rafi Shaik; Associate Professor Rupali Datta; and Wusirika has been published in the January issue of Environmental Science and Technology.